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KMID : 0604520220480030255
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Journal of the Society of Cosmetic Scientists of Korea
2022 Volume.48 No. 3 p.255 ~ p.264
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Optimization of Polyphenol Extraction Process from Native Soybean using Ultrasound
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Kang Hye-Jung
Park Jun-Seong
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Abstract
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The active ingredients of Napttegi Kong(GML, Glycine max landrace), a type of native rare soybeans, were identified, and an ultrasonic extraction method was introduced as an eco-friendly extraction method. Through the component analysis of the Napttegi Kong extract, the epicatechin, which was not found in conventional soybeans, was identified.
For effective extraction using ultrasonic, the main extraction conditions were optimized using the response surface analysis method. Through the Box-Behnken design process, 15 experiments were conducted with the extraction temperature, the ratio of extraction solvent/solution, and extraction time as key independent variables. A quadratic regression equation for the two dependent variables, epicatechin content and total isoflavone content, was derived, and the coefficients of determination were found to be high as R2 = 0.9939 and R2 = 0.9844, respectively, confirming that the correlation showed high significance. The extraction conditions satisfying the maximum expectations of these two dependent variables were predicted. to be 40.4°C of extraction temperature, 19.3 times of extraction solvent/solution, and 91 sec of extraction time.
The expected value and the actual experimental value of the epikatechin content and the total isoflavone content were similar, so it was confirmed that this experimental method is a highly reliable optimization model.
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KEYWORD
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native soybean, epicatechin, ultrasonic, isoflavone, RSM
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